Methods and compositions for diagnostic assays
申请公布号:AU2021400(A)
申请号:AU20000020214
申请日期:1999.11.04
申请公布日期:2000.05.29
发明人:JEFFREY E. W. MILLER
分类号:C12Q1/68
主分类号:C12Q1/68
摘要:A method for detecting a diagnostic marker in a biological sample. In one aspect, the invention comprises providing a biological sample comprising DNA encoding sequences diagnostic for the diagnostic marker, mixing the sample with at least three primers comprising at least two differentially-labeled primers which recognize and bind to at least one intervening DNA sequence region in the DNA that is diagnostic for the diagnostic marker, incubating the mixture under conditions sufficient to result in detectable amplification of the intervening DNA sequence region, and analyzing the products of the amplification step to detect the individual amplification product diagnostic for the diagnostic marker.