METHOD FOR CULTURING CELL TO ENHANCE SECRETION RATE OF RECOMBINANT PROTEIN

申请公布号：KR100251521(B1)

申请号：KR19970058172

申请日期：1997.11.05

申请公布日期：2000.04.15

申请人：

LG CHEMICAL CO.,LTD

发明人：AHN, YONG-HO;HAN, KYU-BUM;YOON, SUNG-KWAN;KWON, INN-CHAN

分类号：C12N1/00;(IPC1-7):C12N1/00

主分类号：C12N1/00

摘要：PURPOSE: Provided is a method for culturing cell to enhance the secretion rate of a recombinant protein. The method can inhibit the repression of activity of a recombinant protein by death or crush of a cell and increase the secretion rate of a protein more than four times. CONSTITUTION: A method for culturing cell to enhance the secretion rate of a recombinant protein is composed of the following steps of: (a) growing a cell sufficiently using a medium including serum for cell growth; and (b) increasing the secretion rate of a recombinant protein using serum free medium including more than one reductant selected form cysteine, glutathione, reduced form, 2-mercaptoethanol, cysteamine or mixture thereof for producing a recombinant protein.

SPECIES-SPECIFIC OLIGONUCLEOTIDES FOR DETECTION OF A QUARANTINE SPECIES, FUSARIUM CULMORUM BY PCR AND THE KIT COMPRISING THE SAME

METHOD OF DATA TRANSMISSION FOR EFFICIENT ADAPTIVE LINK SCHEME

MEANS FOR NON-CONTACT USER INTERFACE, AND DEVICE WITH THE MEANS

APPARATUS FOR STRAIGHTENING METAL STRIP

DEVICE FOR MINIMIZING OXYGEN CONTENT

COMMON MODE FILTER AND METHOD OF MANUFACTURING THE SAME

DEPOSITION APPARATUS

HANDOVER METHOD IN COMMUNICATION SYSTEM BASED ON BEAM DIVISION MULTIPLE ACCESS AND MOBILE STATION PERFORMING THE METHOD

MOBILE PHONE DISPLAY INFORMATION MANAGEMENT METHOD FOR MOBILE PHONE CONNECTION COMPUTER PROGRAM

DI(AMINOGUANIDIUM) 4,4',5,5'-TETRANITRO-2,2'-BIIMIDAZOLE, AND PREPARATION METHOD THEREOF

REGULATOR AND DRIVING APPARATUS OF SATELLITE SEPARATION DEVICE INCLUDING IT

A PICKING DEVICE OF ENABLE SWING

SHIP

APPARATUS FOR CROSS BEAM AND PROCESSING SUBSTRATE

ECCENTRIC BITE

POLYPROPYLENE RESIN AND RESIN COMPOSITION COMPRISING THE SAME

CHIP SUPPLY DEVICE FOR COMPACTOR

IMAGE DISPLAY APPARATUS AND METHOD FOR OPERATING THE SAME

Padding buffer requests to avoid reads of invalid data

System and method for improving cache performance