REPORTER FUSION PROTEINS, EXPRESSION VECTORS AND TRANSFECTED CELL LINES THEREOF FOR THE ANALYSIS OF NUCLEAR TRANSPORT,申请号WO1998CA00385-传众专利搜索

REPORTER FUSION PROTEINS, EXPRESSION VECTORS AND TRANSFECTED CELL LINES THEREOF FOR THE ANALYSIS OF NUCLEAR TRANSPORT

申请公布号：WO9849325(A1)

申请号：WO1998CA00385

申请日期：1998.04.24

申请公布日期：1998.11.05

申请人：

MCGILL UNIVERSITY;STOCHAJ, URSULA

发明人：STOCHAJ, URSULA

分类号：C07K14/435;C07K14/46;(IPC1-7):C12N15/62;C12N15/85;C12N5/10;G01N33/68

主分类号：C07K14/435

摘要：The present invention relates to reporter fusion proteins which allow the monitoring of facilitated transport and passive diffusion of proteins across the nuclear envelope in mammalian cells, cloning and expression vectors encoding these reporter proteins and transfected cell lines thereof. More particularly, the present invention relates to genes encoding reporter proteins to monitor transport and passive diffusion of proteins across the nuclear envelope in mammalian cells, which comprises a protein carrying a nuclear localization sequence (NLS) fused to at least one copy of a reporter protein, wherein the reporter protein allows for the visualization of the reporter fusion protein.