Recombinant protein production with reduced expression of selectable markers,申请号US19930006863-传众专利搜索

Recombinant protein production with reduced expression of selectable markers

申请公布号：US5716803(A)

申请号：US19930006863

申请日期：1993.01.21

申请公布日期：1998.02.10

申请人：

REGENERON PHARMACEUTICALS, INC.

发明人：PANAYOTATOS, NIKOS

分类号：C07K14/525;C12N1/21;C12N15/67;C12N15/70;(IPC1-7):C12P21/00;C12N15/63;C12N15/65;C07H21/04

主分类号：C07K14/525

摘要：The present invention relates to expression of recombinant proteins by use of a bacterial host expression vector which expresses a recombinant protein under the control of a first regulatory expression element, and expresses a selectable marker under the control of a second regulatory expression element, which second element is mutated such that expression of the selectable marker is at reduced levels relative to that directed by such an unmutated expression element. Such an expression vector in a suitable bacterial host (a) allows ease of purification of the recombinant protein of interest ("the recombinant protein") since less selectable marker is present to interfere with the purification of the recombinant protein, and (b) increases the amount of recombinant protein that is produced by the bacterial host cell. Furthermore, in an embodiment in which the selectable marker is an antibiotic resistance gene, expression according to the present invention ensures that only the minimal necessary levels of antibiotic will be used for selection during fermentation. The invention provides expression vectors, host-vector expression systems (comprising the expression vector in a bacterial host in which it can be expressed), and methods relating thereto.