PRODUCTION OF ENZYMATICALLY ACTIVE RECOMBINANT CARBOXYPEPTIDASE B

申请公布号：WO1996023064(A1)

申请号：US1996000995

申请日期：1996.01.25

申请公布日期：1996.08.01

摘要：<p>The subject invention provides a method of producing enzymatically active CPB which comprises treating a recombinant cell containing DNA encoding ProCPB, so that the DNA directs expression of the ProCPB, recovering from the cell the ProCPB so expressed, treating the recovered ProCPB under conditions permitting folding of the ProCPB, subjecting the folded ProCPB to enzymatic cleavage to produce enzymatically active CPB and purifying the enzymatically active CPB.</p>

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