CELL CULTIVATION,申请号JP19900095194-传众专利搜索

CELL CULTIVATION

申请公布号：JPH03292884(A)

申请号：JP19900095194

申请日期：1990.04.12

申请公布日期：1991.12.24

申请人：

MITSUBISHI RAYON CO LTD

发明人：MIYAMORI TAKAO;ENOMOTO KANEHIKO;MATSUMOTO SHIRUYOSHI;SAKIMAE AKIHIRO;NUMAZAWA RYOZO

分类号：C12M3/00;C12N5/07;C12N5/071;C12N5/10

主分类号：C12M3/00

摘要：<p>PURPOSE:To improve adhesiveness, propagability and extensibility, etc., of cell by keeping a specific crosslinked polymer on the surface of at least a part of porous membrane. CONSTITUTION:(A) A polymerizable monomer (e.g. maleic anhydride) having acid anhydride as a functional group is copolymerized with (B) a monomer component containing >=30wt.% divinylbenzene in a molar ratio of B/A=1/1-2/1 to obtain a crosslinked polymer. Next, 7-150wt.% said crosslinked polymer is kept on the surface of at least a part of porous membrane 2 having 0.01-5mum fractionated particle diameter and 10-90% porosity. Then, a culturing region 6 having a cell-adding port 3 and a region 7 having a medium inlet 4 and an outlet 5 are separated by said porous membrane 2, thus the regions 6 and 7 are filled with a liquid medium and necessary numbers of cell are added through the adding port 3, then the medium is flowed in the region 7, thus the medium in the region 6 is adjusted at a fixed temperature to culture cell.</p>