DETECTION OF POLYMORPHIC RESTRICTION SITES USING HYBRIDIZATION PROBE,申请号AU19850043083-传众专利搜索

DETECTION OF POLYMORPHIC RESTRICTION SITES USING HYBRIDIZATION PROBE

申请公布号：AU587189(B2)

申请号：AU19850043083

申请日期：1985.05.29

申请公布日期：1989.08.10

申请人：

CETUS CORPORATION

发明人：RANDALL KEICHI SAIKI;HENRY ANTHONY ERLICH

分类号：G01N33/50;C07H21/04;C12N15/09;C12Q1/68;G01N33/58;(IPC1-7):C12Q1/68

主分类号：G01N33/50

摘要：<p>In a method for detecting the presence or absence of a specific restriction site in a nucleic acid sequence an oligonucleotide probe complementary to one strand of the nucleic acid sequence spanning said restriction site is synthesized. The probe is labeled at the end nearer the restriction site. The nucleic acid is hybridized to the probe and a blocking oligomer may be added, if necessary, to prevent non-specific binding of the probe. Subsequent digestion with a restriction enzyme cleaves those oligomers that have hybridized to the nucleic acid and reformed the restriction site. The resulting cut and uncut labeled oligomers are separated and detected based on the type of probe label. The described method may be used to detect sickle cell anemia.</p>