DNA SEQUENCE CODING SECRETION SIGNAL PEPTIDE

申请公布号：JPH01124384(A)

申请号：JP19870279157

申请日期：1987.11.06

申请公布日期：1989.05.17

申请人：

AGENCY OF IND SCIENCE & TECHNOL

发明人：TOKUNAGA MASAO;HISHINUMA FUMIO

分类号：C12N15/09;C12N15/00;C12N15/62;C12P21/00;C12R1/645;C12R1/865

主分类号：C12N15/09

摘要：PURPOSE:To improve the secretion efficiency of protein produced in yeast cell, by synthesizing a DNA sequence coding an amino acid sequence containing a secretion signal peptide expressed by a specific structural formula. CONSTITUTION:A DNA sequence coding an amino acid sequence containing a secretion signal peptide expressed by formula I is separated from a region coding a signal peptide of 28Kd of a killer toxin protein of pGKL1. The DNA sequence is inserted between a promoter sequence and an exogenic gene coding a prescribed protein on a proper plasmid vector and expressed in the host yeast to effect the secretion of the objective protein in high efficiency. Saccharomyces cerevisiae containing the above plasmid introduced therein can secrete a foreign protein in a culture liquid in high efficiency.

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