PRODUCTION OF POLYPEPTIDE,申请号JP19860070648-传众专利搜索

PRODUCTION OF POLYPEPTIDE

申请公布号：JPS61268193(A)

申请号：JP19860070648

申请日期：1986.03.28

申请公布日期：1986.11.27

申请人：

CHIRON CORP

发明人：ROORENSU ESU KAUSENSU;PATORISHIA EE TEKANPU ORUSON;JIEFURII AARU SHIYASUTAA;JIEIMUZU PII MERIIUEZAA

分类号：C12N15/09;C07K14/16;C07K14/62;C07K14/65;C07K19/00;C12N1/16;C12N1/19;C12N9/02;C12N15/00;C12N15/81;C12P21/00;C12P21/02;C12R1/865

主分类号：C12N15/09

摘要：Novel methods and compositions are provided for enhanced yield of heterologous proteins in fungi. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide, where the two polypeptides are joined by a selectively cleavable linkage. In particular, a sequence coding for superoxide dismutase is joined to another polypeptide of interest at either terminus of the superoxide dismutase in a yeast expression vector under transcriptional control of an active promoter and the vector introduced into a yeast host and the host grown. High yields of the fusion product are obtained in this manner, where the fusion product can be selectively cleaved so as to produce both the superoxide dismutase and the other polypeptide in high yield. The S. cerevisiae strains 2150-2-3 (pYASI1) and AB110 (pYLUIGF2-14) were deposited at the A.T.C.C. on February 27, 1985 and March 19, 1986, respectively; and given accession nos. 20745 and 20796, respectively. CROSS-REFERENCE TO RELATED APPLICATIONS This application is a Continuation of application Serial No. 717 209, filed March 28, 1985, which disclosure is incorporated herein by reference.