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Electrophoresis method for detecting glycosylated hemoglobin in blood

申请公布号:US4351711(A)

申请号:US19810256201

申请日期:1981.04.21

申请公布日期:1982.09.28

申请人:
GELMAN SCIENCES, INC.

发明人:AMBLER, JEFFREY

分类号:G01N27/447;G01N33/72;(IPC1-7):B01D13/02

主分类号:G01N27/447

摘要:In accordance with the invention the glycosylated hemoglobin in a blood sample is electrophoretically separated from the other constituents by including dextran sulphate in the electrophoresis buffer, or by mixing a solution of the dextran sulphate with the blood sample prior to the electrophoresis, such that the blood is in contact with dextran sulphate during the electrophoresis. The presence of the dextran sulphate results in a clear and well defined electrophoretic separation of the glycosylated hemoglobin from the non-glycosylated hemoglobin, thereby enabling accurate glycosylated hemoglobin determination.

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