PRIMER SETS SPECIFIC TO THE OIL-DEGRADING BACTERIUM, NOCARDIA SP. H17-1 AND A MONITORING METHOD OF THE BACTERIUM BY USING THE SAID PRIMER SETS,申请号KR20050068527-传众专利搜索

PRIMER SETS SPECIFIC TO THE OIL-DEGRADING BACTERIUM, NOCARDIA SP. H17-1 AND A MONITORING METHOD OF THE BACTERIUM BY USING THE SAID PRIMER SETS

申请公布号：KR20070013870(A)

申请号：KR20050068527

申请日期：2005.07.27

申请公布日期：2007.01.31

申请人：

KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

发明人：KIM, HEE SIK;YOON, BYUNG DAE;BAEK, KYUNG HWA;OH, HEE MOCK;CHO, DAE HYUN;SONG, JAE JUN;YOON, JUNG HOON;LEE, SEUNG GOO

分类号：C12Q1/68

主分类号：C12Q1/68

摘要：<p>Primer sets specific to an oil-degrading bacterium, Nocardia sp. H17-1 and a monitoring method of the bacterium by using the same primer sets are provided to control and monitor the oil-degrading bacterium applied to the oil-polluted environments, so that purification efficiency of the oil-degrading bacterium is increased. The primer set specific to an oil-degrading bacterium, Nocardia sp. H17-1 comprises (i) the primers of SEQ ID NO:1 and SEQ ID NO:3 or (ii) the primers of SEQ ID NO:2 and SEQ ID NO:3, wherein the oil-degrading bacterium, Nocardia sp. H17-1 is isolated from the oil-polluted soil. The oil-degrading bacterium, Nocardia sp. H17-1 is monitored by performing real time PCR comprising pre-denaturation step, denaturation step, annealing step, extention step and final extention step using the primer set specific to Nocardia sp. H17-1, SYBR green 1 and hot-start polymerase, wherein the hot-start polymerase is Tbr DNA polymerase derived from Thermus brockianus; and the annealing temperature is 55-65 deg. C.</p>