PROCESS FOR PRODUCING PROTEIN WITH REDUCTION OF MANNOSE PHOSPHATE IN THE SUGAR CHAIN AND GLYCOPROTEIN PRODUCED THEREBY,申请号EP20010930179-传众专利搜索

PROCESS FOR PRODUCING PROTEIN WITH REDUCTION OF MANNOSE PHOSPHATE IN THE SUGAR CHAIN AND GLYCOPROTEIN PRODUCED THEREBY

申请公布号：EP1283265(B1)

申请号：EP20010930179

申请日期：2001.05.16

申请公布日期：2009.08.05

申请人：

MITSUBISHI TANABE PHARMA CORPORATION

发明人：MIURA, MASAMI;HIROSE, MASAAKI;MIWA, TAEKO;IRIE, HIROYUKI;KUWAE, SHINOBU;MIYANO, KENMI;OTANI, WATARU;OHI, HIDEYUKI

分类号：C12N15/52;C07K14/46;C07K16/18;C12N1/19;C12N9/12;C12P21/00;C12P21/02;C12R1/84

主分类号：C12N15/52

摘要：The present invention intends to find out a gene participating in addition of mannose phosphate to a sugar chain of a glycoprotein originating in a yeast belonging to the genus Pichia and provide a means of controlling the same. The present invention also intends to provide a process for producing a protein with reduction of an acidic sugar chain by using the thus controlled yeast strain belonging to the genus Pichia. Namely, the present invention includes a protein participating in the addition of mannose phosphate to a sugar chain of a glycoprotein; a gene coding this protein; a mutant of this gene; a vector carrying the mutant gene; a yeast strain belonging to the genus Pichia having been transformed by this vector; a process for producing a protein with reduction of an acidic sugar chain by using the transformed yeast strain; and a glycoprotein thus produced.